Kirsebom labb

Vår forskningsintresse är biologin av RNA och dess roll i olika cellulära processer.

Beskrivning

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Populärvetenskaplig presentation

Vår forskning har tre huvudinriktningar med syfte att förstå A) mykobakteriernas cellmorfologi och fysiologi, B) små regulatoriska och icke-kodande RNA molekylers funktion hos mykobakterier samt C) den underliggande mekanismen hur ett katalytiskt RNA klyver sitt substrat.

Ett flertal mykobakterier orsakar svåra infektionssjukdomar t ex Mycobacterium tuberculosis som orsakar tuberkulos. Behandling av tuberkulos är lång vilket även gäller andra mykobakterieinfektioner och vi ser en ökad förekomst av antibiotikaresistens. Våra studier kring cellens form och hur denna regleras samt sökandet efter ny kunskap om små RNA molekyler hos mykobakterier kommer att leda till ny kunskap som vi förespeglar oss kommer att få betydelse för den framtida hälsovården och specifikt för behandling av sjukdomar orsakade av mykobakterier.

Forskning

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Gruppmedlemmar

Forskningsledare: Leif Kirsebom

Publikationer

Asymmetric growth and division in Mycobacterium spp.: compensatory mechanisms for non-medial septa

Singh, Bhupender; Nitharwal, Ram Gopal; Ramesh, Malavika; Pettersson, B. M. Fredrik et al.

Ingår i Molecular Microbiology, s. 64-76, 2013
- DOI för Asymmetric growth and division in Mycobacterium spp.: compensatory mechanisms for non-medial septa
Characterization of three Mycobacterium spp. with potential use in bioremediation by genome sequencing and comparative genomics

Das, Sarbashis; Pettersson, B M Fredrik; Behra, Phani Rama Krishna; Ramesh, Malavika et al.

Ingår i Genome Biology and Evolution, s. 1871-1886, 2015
- DOI för Characterization of three Mycobacterium spp. with potential use in bioremediation by genome sequencing and comparative genomics
- Ladda ner fulltext (pdf) av Characterization of three Mycobacterium spp. with potential use in bioremediation by genome sequencing and comparative genomics
Cleavage mediated by the catalytic domain of bacterial RNase P RNA

Wu, Shiying; Kikovska, Ema; Lindell, Magnus; Kirsebom, Leif A.

Ingår i Journal of Molecular Biology, s. 204-214, 2012
- DOI för Cleavage mediated by the catalytic domain of bacterial RNase P RNA
Cleavage mediated by the P15 domain of bacterial RNase P RNA

Kikovska, Ema; Wu, Shiying; Mao, Guanzhong; Kirsebom, Leif A.

Ingår i Nucleic Acids Research, s. 2224-2233, 2012
- DOI för Cleavage mediated by the P15 domain of bacterial RNase P RNA
Cleavage of Model Substrates by Arabidopsis thaliana PRORP1 Reveals New Insights into Its Substrate Requirements

Mao, Guanzhong; Chen, Tien-Hao; Srivastava, Abhishek S.; Kosek, David et al.

Ingår i PLOS ONE, 2016
- DOI för Cleavage of Model Substrates by Arabidopsis thaliana PRORP1 Reveals New Insights into Its Substrate Requirements
- Ladda ner fulltext (pdf) av Cleavage of Model Substrates by Arabidopsis thaliana PRORP1 Reveals New Insights into Its Substrate Requirements
Comparative genome analysis of Mycobacteria focusing on tRNA and non-coding RNA

Behra, Phani Rama Krishna; Pettersson, B. M. Fredrik; Ramesh, Malavika; Das, Sarbashis et al.

Ingår i BMC Genomics, 2022
- DOI för Comparative genome analysis of Mycobacteria focusing on tRNA and non-coding RNA
- Ladda ner fulltext (pdf) av Comparative genome analysis of Mycobacteria focusing on tRNA and non-coding RNA
Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA

Behra, Phani Rama Krishna; Pettersson, Fredrik; Das, Sarbashis; Dasgupta, Santanu et al.

Ingår i BMC Evolutionary Biology, 2019
- DOI för Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA
- Ladda ner fulltext (pdf) av Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA
Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection

Pettersson, B. M. Fredrik; Das, Sarbashis; Behra, Phani Rama Krishna; Jordan, Heather R. et al.

Ingår i PLOS ONE, 2015
- DOI för Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection
Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain

Mao, Guanzhong; Srivastava, Abhishek S.; Wu, Shiying; Kosek, David et al.

Ingår i PLOS ONE, 2018
- DOI för Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain
- Ladda ner fulltext (pdf) av Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain
Cross talk between the +73/294 interaction and the cleavage site in RNase P RNA mediated cleavage.

Brännvall, Mathias; Kikovska, Ema; Kirsebom, Leif

Ingår i Nucleic Acids Res, s. 5418-29, 2004
De novo search for non-coding RNA genes in the AT-rich genome of Dictyostelium discoideum: Performance of Markov-dependent genome feature scoring

Larsson, Pontus; Hinas, Andrea; Ardell, David H.; Kirsebom, Leif A. et al.

Ingår i Genome Research, s. 888-899, 2008
- DOI för De novo search for non-coding RNA genes in the AT-rich genome of Dictyostelium discoideum: Performance of Markov-dependent genome feature scoring
Differential role of the intermolecular base-pairs G292-C(75) and G293-C(74) in the reaction catalyzed by Escherichia coli RNase P RNA.

Busch, S; Kirsebom, Leif; Notbohm, H; Hartmann, R K

Ingår i J Mol Biol, s. 941-51, 2000
Differentiation and Phylogenetic Relationships in Mycobacterium spp with Special Reference to the RNase P RNA Gene rnpB

Herrmann, Björn; Stolt, Pelle; Abdeldaim, Guma; Rubin, Carl-Johan et al.

Ingår i Current Microbiology, s. 634-639, 2014
- DOI för Differentiation and Phylogenetic Relationships in Mycobacterium spp with Special Reference to the RNase P RNA Gene rnpB
Escherichia coli ribonuclease P.

Kirsebom, Leif

Ingår i Methods Enzymol, s. 77-92, 2001
Eukaryotic RNase P RNA mediates cleavage in the absence of protein

Kikovska, Ema; Svärd, Staffan; Kirsebom, Leif

Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. 2062-2067, 2007
- DOI för Eukaryotic RNase P RNA mediates cleavage in the absence of protein
Evidence for induced fit in bacterial RNase P RNA-mediated cleavage

Brännvall, Mathias; Kikovska, Ema; Wu, Shiying; Kirsebom, Leif A.

Ingår i Journal of Molecular Biology, s. 1149-1164, 2007
- DOI för Evidence for induced fit in bacterial RNase P RNA-mediated cleavage
Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains

Behra, Phani Rama Krishna; Das, Sarbashis; Pettersson, B. M. Fredrik; Shirreff, Lisa et al.

Ingår i Scientific Reports, 2019
- DOI för Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
- Ladda ner fulltext (pdf) av Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
Extensive genomic diversity among Mycobacterium marinum strains revealed by whole genome sequencing

Das, Sarbashis; Pettersson, B. M. Fredrik; Behra, Phani Rama Krishna; Mallick, Amrita et al.

Ingår i Scientific Reports, 2018
- DOI för Extensive genomic diversity among Mycobacterium marinum strains revealed by whole genome sequencing
- Ladda ner fulltext (pdf) av Extensive genomic diversity among Mycobacterium marinum strains revealed by whole genome sequencing
Functional Coupling between a Distal Interaction and the Cleavage Site in Bacterial RNase-P-RNA-Mediated Cleavage

Wu, Shiying; Chen, Yu; Lindell, Magnus; Mao, Guanzhong et al.

Ingår i Journal of Molecular Biology, s. 384-396, 2011
- DOI för Functional Coupling between a Distal Interaction and the Cleavage Site in Bacterial RNase-P-RNA-Mediated Cleavage
Gene miaA for post-transcriptional modification of tRNA_XXA is important for morphological and metabolic differentiation in Streptomyces

Koshla, Oksana; Yushchuk, Oleksandr; Stash, Iryna; Dacyuk, Yuriy et al.

Ingår i Molecular Microbiology, s. 249-265, 2019
- DOI för Gene miaA for post-transcriptional modification of tRNA_XXA is important for morphological and metabolic differentiation in Streptomyces
Identification and expression of stressosomal proteins in Mycobacterium marinum under various growth and stress conditions

Pettersson, B. M. Fredrik; Nitharwal, Ram Gopal; Das, Sarbashis; Behra, Krishna P. R. et al.

Ingår i FEMS Microbiology Letters, s. 98-105, 2013
- DOI för Identification and expression of stressosomal proteins in Mycobacterium marinum under various growth and stress conditions
Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage

Mao, Guanzhong; Srivastava, Abhishek S.; Wu, Shiying; Kosek, David et al.

Ingår i Scientific Reports, 2023
- DOI för Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage
- Ladda ner fulltext (pdf) av Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage
Importance of the +73/294 interaction in Escherichia coli RNase P RNA substrate complexes for cleavage and metal ion coordination.

Brännvall, Mathias; Pettersson, B M Fredrik; Kirsebom, Leif

Ingår i J Mol Biol, s. 697-709, 2003
Inhibition of Bacterial RNase P RNA by Phenothiazine Derivatives

Wu, Shiying; Mao, Guanzhong; Kirsebom, Leif A.

Ingår i Biomolecules, 2016
- DOI för Inhibition of Bacterial RNase P RNA by Phenothiazine Derivatives
- Ladda ner fulltext (pdf) av Inhibition of Bacterial RNase P RNA by Phenothiazine Derivatives
Inhibition of Klenow DNA polymerase and poly(A)-specific ribonuclease by aminoglycosides

Ren, Yan-Guo; Martínez, Javier; Kirsebom, Leif; Virtanen, Anders

Ingår i RNA, s. 1393-1400, 2002
- DOI för Inhibition of Klenow DNA polymerase and poly(A)-specific ribonuclease by aminoglycosides
Inhibition of poly(A) polymerase by aminoglycosides

Thuresson, Ann-Charlotte; Kirsebom, Leif A.; Virtanen, Anders

Ingår i Biochimie, s. 1221-1227, 2007
- DOI för Inhibition of poly(A) polymerase by aminoglycosides
Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members

Behra, Phani Rama Krishna; Pettersson, Fredrik; Ramesh, Malavika; Dasgupta, Santanu et al.

Ingår i Scientific Reports, 2019
- DOI för Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members
- Ladda ner fulltext (pdf) av Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members
Intracellular localization of the mycobacterial stressosome complex

Ramesh, Malavika; Nitharwal, Ram Gopal; Behra, Phani Rama Krishna; Pettersson, B. M. Fredrik et al.

Ingår i Scientific Reports, 2021
- DOI för Intracellular localization of the mycobacterial stressosome complex
- Ladda ner fulltext (pdf) av Intracellular localization of the mycobacterial stressosome complex
Sidney Altman: A brief memoir

Kirsebom, Leif A.

Ingår i RNA, s. 1404-1405, 2022
Sporulation in mycobacteria

Ghosh, Jaydip; Larsson, Pontus; Singh, Bhupender; Pettersson, B. M. Fredrik et al.

Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. 10781-10786, 2009
- DOI för Sporulation in mycobacteria
Substrate discrimination in RNase P RNA-mediated cleavage: importance of the structural environment of the RNase P cleavage site.

Kikovska, Ema; Brännvall, Mathias; Kufel, Joanna; Kirsebom, Leif

Ingår i Nucleic Acids Res, s. 2012-21, 2005
The Mycobacterium phlei Genome: Expectations and Surprises

Das, Sarbashis; Pettersson, B. M. Fredrik; Behra, Phani Rama Krishna; Ramesh, Malavika et al.

Ingår i Genome Biology and Evolution, s. 975-985, 2016
- DOI för The Mycobacterium phlei Genome: Expectations and Surprises
- Ladda ner fulltext (pdf) av The Mycobacterium phlei Genome: Expectations and Surprises
The Presence of a C_− 1/G_+ 73 Pair in a tRNA Precursor Influences Processing and Expression In Vivo

Pettersson, B. M. Fredrik; Kirsebom, Leif A.

Ingår i Journal of Molecular Biology, s. 1089-1097, 2008
- DOI för The Presence of a C_− 1/G_+ 73 Pair in a tRNA Precursor Influences Processing and Expression In Vivo
The residue immediately upstream of the RNase P cleavage site is a positive determinant.

Brännvall, Mathias; Fredrik Pettersson, B M; Kirsebom, Leif

Ingår i Biochimie, s. 693-703, 2002
Transition-state stabilization in Escherichia coli ribonuclease P RNA-mediated cleavage of model substrates

Wu, Shiying; Chen, Yu; Mao, Guanzhong; Trobro, Stefan et al.

Ingår i Nucleic Acids Research, s. 631-642, 2014
- DOI för Transition-state stabilization in Escherichia coli ribonuclease P RNA-mediated cleavage of model substrates
tRNA accumulation and suppression of the bldA phenotype during development in Streptomyces coelicolor

Pettersson, B M Fredrik; Kirsebom, Leif A

Ingår i Molecular Microbiology, s. 1602-1614, 2011
- DOI för tRNA accumulation and suppression of the bldA phenotype during development in Streptomyces coelicolor